“Biotics” (which means relating to or resulting from living things) is a term we have come to hear a lot about over the past few decades and has increasingly been used to describe a plethora of different health-promoting ingredients relating to beneficial microorganisms.
Prebiotics, probiotics, synbiotics and postbiotics – what do each of these do and how are they different from each other? All of these play an important role to your gut microbiome and to your overall health.
Let’s start with what the gut microbiome is. According to the Encyclopedia of Cell Biology, the gut microbiome is “the collection of microorganisms including bacteria, archaea [also known as ‘the third domain of life’], viruses, and fungi found within the gut and their overall genetic information.” A flourishing and balanced microbiome is key to optimum health.
We’ve put together some summaries of how scientists are defining the different “biotic” categories and what that means:
The definition of probiotics as set forth by the FAO and the World Health Organization (WHO) is generally accepted and acknowledged by the majority of the scientific community and is endorsed by the International Scientific Association for Probiotics and Prebiotics (ISAPP).
- They agree that probiotics are “live microorganisms which when administered in adequate amounts confer a health benefit on the host.”
Let’s unpack this definition together. First, probiotics, must be microorganisms. Probiotics, therefore, can include organisms other than just bacteria from the well-known Lactobacillus or Bifidobacterium genera. Bacteria, fungi, or protozoa from any genera can technically be probiotics provided they also meet the other requirements of the FAO/WHO definition. Spore-forming soil bacteria (like Bacillus subtilis), novel yeasts (like Saccharomyces boulardii), and bacteria commonly found in the healthy gut (like Akkermansia muciniphila and Faecalibacterium prausnitzii) are all examples of probiotic potential.
Secondly, probiotics must be live. If microorganisms are dead at the time of use or before they reach the site of action in the body, no matter how effective they are, they cannot be called probiotics. There are many microorganisms that cannot currently be considered probiotics simply because they cannot be kept alive during manufacture or storage. Many of the potentially beneficial bacteria that naturally live in the healthy human gut (e.g., Akkermansia muciniphila and Facalibacterium prausnitzii) have not been commercialized as probiotics because of their extreme sensitivity to oxygen and other stressors. This sensitivity makes them die when manufactured and stored under typical conditions. Even many common Lactobacillus and Bifidobacterium strains die-off when exposed to heat, certain pHs, and/or moisture during storage. There is, and has been, considerable time, research, and money invested in processes, coatings, capsules, and even packaging technology to try and protect these bacteria from the stressors of manufacture, storage, and gastric transit. Other microorganisms, like spore-forming Bacillus and yeast, are naturally stress-tolerant and therefore easier to keep alive during manufacture and storage.
Lastly, probiotics must “confer a health benefit on the host” when “administered in adequate amounts.” To know what an “adequate amount” is, a microorganismal strain must be tested in a clinical trial. Without clinical trial data, any assumption around a successful dose is really just speculation. Clinical trials are also necessary to establish what, if any, “benefit on the host” can be conferred by a microorganism.
Even though ISAPP published a consensus statement on the definition of a prebiotic in 2017, there is still some debate in the scientific and regulatory communities around what can be considered a prebiotic.
- The ISAPP definition states that a prebiotic is “a substrate that is selectively utilized by host microorganisms conferring a health benefit.”
- Predating the ISAPP consensus, the Food and Agriculture Organization of the United Nations (FAO) defines a prebiotic as a “nonviable food component that confers a health benefit on the host associated with modulation of the microbiota.”
- The FDA has historically cited a different definition as a “non-digestible food ingredient that beneficially affects the host by selectively stimulating the growth and/or activity of one or a limited number of bacteria in the colon, and thus improves host health.”
What is the recurring theme? Most of these definitions imply that a prebiotic must be a non-living substance that is undigestible by the intended human or animal consumer and that they must improve host health through selective benefit to certain ‘good’ microorganisms (also known as probiotics) in or on the host.
Non-digestible oligosaccharides (like lactose, or human milk) that can be selectively fermented by ‘good’ bacteria can be considered prebiotics provided they, via clinical trial, have been proven to confer “a health benefit” on the host. But prebiotics do not have to be oligosaccharide or starch based. For example, it has been hypothesized that certain polyphenols, which are abundant in plants, or conjugated fatty acids, which are determined by meat product processing, can also be selectively utilized by certain commensal microbiota.
Importantly, prebiotics do not need to be fermented by ‘good’ microorganisms—aka ‘good’ microorganisms don’t necessarily have to “eat” prebiotics—to selectively benefit them. Polyphenols may also selectively benefit certain ‘good’ microorganisms and moderate the overall gut microbiome by inhibiting specific ‘undesirable’ bacteria. Novel phage-based probiotics can destabilize ‘undesirable’ bacteria thereby releasing the nutrients therein for selective feeding by ‘good’ microorganisms. In doing so, phage-based prebiotics also benefit ‘good’ microorganisms by opening up valuable host real-estate for further colonization.
There is also some debate on the definition of synbiotics.
- ISAPP defines a synbiotic as a “mixture comprising live microorganisms and substrate(s) selectively utilized by host microorganisms that confers a health benefit on the host.” In practice this generally refers to a combination of probiotics and prebiotics, but according to the ISAPP definition, that doesn’t necessarily have to be the case.
- Other more strict definitions in scientific literature include: a “mixture of probiotics and prebiotics that beneficially affects the host by improving the survival and activity of beneficial microorganisms in the gut.”
Synbiotics can be complementary, meaning that their benefit is simply in addition to the probiotic and the prebiotic. This can often be the case when the probiotic in the mixture does not utilize or benefit from the prebiotic in the mixture. Synbiotics can also be synergistic. There is increasing interest in synergistic synbiotics as their benefit is, by definition, greater than the sum of the parts. As with other biotics, the benefit of synbiotics should be demonstrated by clinical trial.
Postbiotics are the newest in popularity and the most convoluted in terms of definition.
According to ISAPP’s recent published definition:
- A postbiotic is a “preparation of inanimate microorganisms and/or their components that confers a health benefit on the host.”
Based on this definition, a postbiotic must be made of dead microorganisms or a biomass from dead microorganisms, like cell wall components or flagella. These alone or in combination with metabolites produced by these microorganisms before being killed can be considered postbiotics. As with ISAPP’s definition of prebiotics and synbiotics, there is also disagreement on this definition especially since many have historically used the term to describe just metabolites or products of fermentation (like enzymes and short chain fatty acids), not the dead microorganisms themselves. In either case, postbiotics cannot be living and must include a component or product from the once living microorganism. Postbiotics must also confer a health benefit, which as has been stated before, can only be proven via clinical trial.
Deerland Probiotics and Enzymes
Deerland Probiotics and Enzymes specializes in solutions that benefit microbiome health using probiotics, prebiotic, and enzyme technology. We help companies with their product lines and understand that you make products for people of different ages, eating habits, dietary restrictions, and cultures. We’re excited to help you achieve a formulation that fits the performance needed for your customers.
We have a team of industry experts working in a state-of-the-art lab and are positioned to help our customers from the initial stages of product conceptualization to final formulation and contract manufacturing.
Deerland Probiotics & Enzymes has announced the publication of a new clinical study examining the immune support effects of Bacillus subtilis DE111®.
The study, “Examining the Gastrointestinal and Immunomodulatory Effects of the Novel Probiotic Bacillus subtilis DE111” published in the International Journal of Molecular Sciences, is an IRB-approved, randomized, double-blind, placebo-controlled study involving 44 healthy adults (aged 20 to 62). Participants consumed either B. subtilis DE111® (1 billion CFU) or placebo daily for four weeks. The researchers examined peripheral blood mononuclear cells (PBMCs) at basal levels pre- and post-study, as well as in response to stimulation with bacterial lipopolysaccharide (LPS), which encourages neutrophils, macrophages and other innate immune cells to release pro-inflammatory cytokines. The results of the study indicated that DE111 can support a healthy immune system and a robust immune response.
After only four weeks of supplementing with DE111, the authors found, participants displayed immunomodulatory and anti-inflammatory effects on several T cell subsets. “This is supported by the observed reduction in immune cell populations within the basal state,” they write. “The downregulation of these cell types in circulation may implicate an overall anti-inflammatory state demonstrated by the decrease in peripheral surveillance and effector cell types.” Another proposed mechanism suggests that DE111 ingestion improved the gut barrier function resulting in less circulating LPS, thereby reducing inflammation levels. In addition, the authors found those in the DE111 group exhibited an increase in anti-inflammatory immune cell populations in response to ex vivo LPS stimulation of PBMCs, which is indicative of a more robust response to prime the immune system. In summary, they explained, this study shows supplementing with B. subtilis DE111 “modulates the host immune system through anti-inflammatory and suppressive action on both innate and adaptive cell types.”
“This newly published study substantiates the efficacy of B. subtilis DE111® for use in products that address the most common concerns of the day– a strong immune system with a quick response to foreign invaders,” said Dr. John Deaton, vice president of science and technology at Deerland Probiotics & Enzymes. “The latest publication adds to an impressive list of clinical studies showing DE111’s impact on digestive, children’s and cardio health and sports performance/recovery,” Deaton adds.
In a previous article, we covered how probiotics like Bacillus subtilis DE111® contribute to effective sports nutrition and help athletes achieve better performance and feel their best – but that’s only half of the total picture.
Enzyme supplements are also a critical part of a well-rounded athlete’s health, as they aid their bodies in achieving more effective protein digestion. That enhanced digestion brings a wide range of benefits.
Enzymes’ Role in Elevating Protein Digestion
Proteins, by their most basic definition, are large chains of amino acids. For both naturally occurring protein and that found in supplements taken by athletes to enhance muscle growth and more, those amino acids need to be broken down and absorbed by the body.
The problem is that digestion must occur within 90 minutes for the body to absorb amino acids and begin reaping their benefits. If proteins aren’t broken down within that timeframe, they’re excreted, causing the amino acids to be wasted and resulting in digestive discomfort from large, undigested peptides.
This means that athletes’ bodies, in particular, need efficient ways to make that happen and ensure optimal protein absorption.
Enzyme-powered sports supplements can provide a much-needed assist to ensure athletes are making the most of the protein that enters their bodies.
ProHydrolase® Brings Athletes Superior Protein Digestion
Deerland’s ProHydrolase® solution is clinically proven to deliver athletes those key benefits in protein digestion.
When combined with popular protein supplements like whey powder, it helps digest protein into a bio-usable form and has been clinically proven to increase branch-chained amino acids, or BCAAs, in the blood by as much as 20%.
By decreasing levels of inflammation-causing C-reactive proteins, assisting athletes’ bodies in absorbing more protein rather than excreting it, and reducing digestive discomfort, ProHydrolase® enables athletes ingesting whey, soy, egg, casein, pea and hemp proteins both naturally and through supplements to feel and perform their best.
Athletic performance comes back to an athlete’s ability to grow stronger, build muscle, and recover more quickly, and ProHydrolase® helps unlock the path toward all of those goals.
Enzyme Supplement Delivery: A Wide Range of Options
Because ProHydrolase® functions as an ingredient that can be leveraged on its own or used in custom formulations, there are a variety of ways in which athletes can take advantages of the benefits it brings.
One IRONMAN athlete formerly held back by digestive upset makes post-workout smoothies with a protein blend featuring ProHydrolase®, eliminating that discomfort without sacrificing the benefits of protein supplements.
Another looking to gain muscle mass found similar success, while yet another has even noticed measurable benefits over plant-based proteins touted to provide similar benefits in terms of better absorption and reduced upset.
To learn more about where to find ProHydrolase®, its benefits and more, contact Deerland today.
From a person’s first venture into the world of athletics to professionals at the height of their game, sports offer a way to have fun, engage in healthy competition, remain fit and build lasting memories.
However, to reap those benefits, athletes need to keep their bodies in optimal condition. Training, diet and mindset go a long way – but they may not always be enough.
The Need for Probiotics to Round Out Sports Readiness and Recovery
The health of a person’s microbiome and digestive tract – in simpler terms, their gut – is critical to gaining a competitive edge via holistic fitness.
Probiotic supplements specifically target that aspect of an athlete’s wellness, increasing gut health and elevating overall athletic performance.
Probiotics boost the health of athletes in three key ways:
- Digestion Optimization
In order to have optimal digestion, your body needs to absorb nutrients, digest proteins and, overall, work to provide the building blocks for both the building of muscle and recovery after exertion. Probiotic supplements aid athletes’ bodies in all three categories, increasing absorption and digestion capabilities to provide that solid foundation for performance.
- Immunity Strengthening
Similar to digestion optimization, athletes’ bodies also need a strong immune system to keep them on the field – and probiotics help them do just that. The cells responsible for immune strength are in the gut, and probiotics work to support those cells. When athletes are less susceptible to sickness and infection, even small upsets, they’re better able to perform at the peak of their ability.
- GI Issue Minimization
Every athlete has likely experienced it – after all their hard work and extensive training, something as seemingly inconsequential as gas, diarrhea, bloating, heartburn or other gastrointestinal issues can derail that progress. Probiotic supplements minimize the risk and effects of these barriers to performance, leaving athletes free and clear to do their best work.
How Do Probiotic Supplements Bring these Benefits?
Effective probiotic supplements, such as Deerland’s Bacillus subtilis DE111, work in a variety of ways to deliver athletes’ bodies the aforementioned benefits.
DE111 produces enzymes that aid in the digestion of proteins and the uptake of nutrients, and also helps produce short-chain fatty acids that further boost digestive capabilities and the immune system. Clinical studies have shown the beneficial effects of combining DE111 with a protein drink on athletes’ performance in the weight room, body composition and recovery.
Contact Deerland Today
Deerland Probiotics & Enzymes has worked to develop science-backed ingredients that support the demands of active individuals, and industry-leading products have come out of that effort, including the probiotic DE111.
To learn more, contact Deerland today.
The market of products containing probiotic and prebiotic supplements is unlike any time in history.
No longer confined to a pill or yogurt, there is a wide swath of products available for consumers concerned about their gut health. Noah Voreades, managing director for GenBiome Consulting, says products like Olipop soda or probiotic gummies on the market not only are expanding the demographics of those using products to help gut health but also changing ideas of probiotics across generations.
“I think a perfect example is college students, which can fall anywhere from the older edge of Gen Z to younger millennials, they buy products now that they’re home during COVID, having to educate themselves remotely. They’re having interactions with their parents, their parents are seeing them consuming new and novel products that they find really efficacious that taste good, that meet their lifestyle and there’s this cross-mingling.”
That sort of experience can encourage members of the baby boomer generation, who my be fatigued by taking a number of pills, to utilize microbiome-based products in a non-traditional form.
Beyond simply taking age and taste into consideration, the microbiome market is drilling down even more on the individual.
“The reason why personalization is entering the microbiome product space is because consumers, we’re becoming used to things being designed, built experienced around us,” said Voreades, citing algorithms used by Netflix or Spotify. “That’s bleeding into the drive to make microbiome products more personalized. Also, I think foundationally when we look at the science and the state of the science in the microbiome space, we’re starting to realize that everyone’s microbiome is very unique. There’s a lot of variation between you and I.”
The advances in technology will be fascinating to follow as science continues to dedicate serious resources to exploring gut health and how it can be improved across age groups and other demographic groups.
Deerland Probiotics and Enzymes has announced the publication of a clinical study demonstrating efficacy of the probiotic Bacillus subtilis DE111® in promoting healthy endothelial function and support of healthy cholesterol levels.
The study, “Bacillus subtilis DE111 intake may improve blood lipids and endothelial function in healthy adults” published in Beneficial Microbes, was performed at a leading university specializing in probiotics research and is an IRB-approved, randomized, double-blind, placebo-controlled four-week trial involving 43 participants aged 18 to 65.
The overall goal of this parallel arm trial was to determine if various cardiovascular parameters in healthy adults could be altered by probiotic supplementation. This new research was based on findings from previous studies that showed clear correlations between certain probiotic strains and healthy vascular function, reduced systemic inflammation and glucose tolerance, as well as evidence that the species Bacillus subtilis can support healthy cholesterol levels with positive cardiovascular effects in animal models.
The research team found that B. subtilis DE111 supplementation of 1 billion CFU per day resulted in significant reduction in total cholesterol and non-HDL cholesterol relative to baseline measures. The team also observed a strong trend toward reduction in LDL cholesterol, as well as improvement in endothelial function; reactive hyperemia index (RHI), an indicator of blood flow and heart health, increased by 9.14%.
“To our knowledge, this is the first randomized controlled trial to investigate the effects of B. subtilis supplementation on these parameters in a human population,” the principal investigators of the study stated. The authors of the study believe that B. subtilis supplementation may have an indirect effect on enhancing the generation of short chain fatty acids (SCFA) leading to this cholesterol lowering effect.
“Notably, these effects were observed in a population of healthy individuals, and the results strongly suggest that B. subtilis supplementation may help support cardiovascular health,” commented Dr. John Deaton, vice president of science and technology at Deerland.
Deaton added that the spore-forming properties of the probiotic DE111® allow formulating in a wide array of attractive consumer products even beyond traditional supplement capsules, including gummies, chocolates, functional beverages and fitness nutrition powders.
There’s a “new” hot topic in gut health. While the concept of “synbiotics” was first proposed more than two decades ago, the term is coming back into the collective supplement lexicon in a big way.
So, what are synbiotics, and what impact will they have on the probiotics market and the way consumers use and view supplements?
Probiotics are live, beneficial bacteria that help contribute to a healthy gut, while prebiotics are indigestible substrates that help “good” bacteria grow and thrive. Synbiotics, then, refer to the practice of combining the two into a single promoter of good digestive health.
However, this definition could also be limiting. Instead, a panel of experts labeled synbiotics “a mixture comprising live microorganisms and substrate(s) selectively utilized by host microorganisms that confers a health benefit on the host.”
Essentially, synbiotics are a combination of “good” bacteria and the things that help keep those bacteria alive, with the end goal of delivering health benefits to consumers.
Synbiotics and Their Market Share Are Growing
While probiotics have traditionally dominated microbiome support sales, prebiotics and synbiotics are gaining ground.
In particular, synbiotics are expected to continue eclipsing $2.5 billion in sales each year through 2023, according to Nutrition Business Journal, and the overall growth percentage of both prebiotics and synbiotics will continue to outpace probiotics.
This growth could come from a variety of consumer motivations, including better education and a desire for more sophisticated gut-health solutions. According to a New Hope Network NEXT Data and Insights survey, consumers are increasingly concerned with immunity, which now lands alongside general health and wellness and vitamin intake as key drivers for supplement use.
In short, consumers are looking for supplements that deliver wholistic health benefits, and with results backed by science and measurable impact.
How PreforPro® Delivers Synbiotic Benefits
Deerland’s novel prebiotic PreforPro®, a blend of bacteriophages, exhibits synbiotic benefits when combined with probiotic supplementation, suggesting synergistic benefits for users.
In fact, a study designed to test PreforPro’s efficacy in delivering these synbiotic benefits “observed improvements in GI inflammation and a trending improvement in colon pain in individuals consuming B. lactis with PreforPro, but not in the group consuming only the probiotic.” In addition, a significant increase in Lactobacillus species was observed, demonstrating that supplementing the probiotic B. lactis with PreforPro can significantly modulate gut ecology to favor beneficial bacteria.
This means that PreforPro® proved more effective than a probiotic alone, suggesting that “the addition of PreforPro® as a combination therapy may alter gut ecology to extend the GI benefits of consuming B. lactis or other probiotics.”
To learn more about PreforPro® and Deerland’s other solutions for microbiome health, contact us today.
Probiotics are known as “good” or “beneficial” bacteria. Probiotic supplements and foods rich in them can deliver many benefits for the human body. Dozens of different probiotic bacteria contribute to improved health.
Most people are aware of how they help the digestive system, but probiotics impact other aspects of health, as well. With this in mind, the product development specialists at Deerland stand ready to provide turnkey product formulations solutions, as well as help with developing customized, condition-specific formulas with a probiotic base. Let’s look at the benefits of probiotics for sleep, mood and the immune system.
Probiotics and Better Sleep
Everyone wants more restorative sleep. According to the American Sleep Association (ASA), 50 to 70 million adults have a sleep disorder. Insomnia is the most common.
Because of the prevalence of inadequate sleep, many seek treatments, including taking supplements. A specially formulated probiotic for rest could provide relief. An example sleep support with probiotics formula may include:
- Probiotic Bacillus subtilisDE111®: It aids in keeping intestines healthy and digestion normal. If the digestive system works efficiently, it’s much easier to find restful sleep.
- Prebiotic PreforPro®: This ingredient may relieve occasional stomach discomfort, as it stimulates beneficial bacteria growth in the gut.
- Melatonin: This natural hormone works through the endocrine system, supporting the sleep/wake cycle of the circadian rhythm.
- Herbs and Botanicals known for supporting sleep, such as lemon balm extract, chamomile or valerian root:
Probiotics in combination with botanical blends and other functional ingredients can promote healthy sleep, often making it more restorative.
Probiotics and Mood
Improving mood could start with the right probiotic supplement. Adding vitamins and amino acids in the formula encourages a normal, healthy mood. A good mood support with probiotics formula may consist of:
- Bacillus subtilisDE111®: DE111 aids in the growth of good bacteria in the gut while out-competing undesirable bacteria for improved digestion. Some intestinal microbiota can secrete neurotransmitters. Increased amounts of good bacteria can have a positive impact on mood.
- Bifidobacterium longum: This probiotic strain features in several studiesfocusing on the effects of probiotic supplementation on mood state. Those studies propose that probiotics may improve psychological well-being by ameliorating aspects of mood and sleep quality, reinforcing the theory of the gut-brain axis.
- Solarplast®: This spinach extract contains photosynthetic complexes of energy molecules, vitamins and powerful antioxidants. They spark cellular energy production, which the body needs for all systems. The high antioxidant content may work to contest free radical damage, a cause of neuroinflammation. This can directly impact mood.
- Additional vitamins and amino acids known to support a healthy mood, such as Vitamin D3 and L-theanine
Probiotics and the Immune System
A probiotic supplement with additional immune support ingredients can be very beneficial to the body. Probiotics already support gut health, which is critical to the immune system. A probiotic formula to support immune health could include:
- Spore-forming probiotic bacteria with scientifically researched immune health benefits, such as B. subtilis DE111®, B. coagulans, and B. clausii
- Solarplast®: This ingredient delivers detoxifying antioxidants, which can slow cell damage caused by free radicals. It contains glutathione and its enzyme partners that accelerate antioxidant reactions.
- Immune health-supporting vitamins, minerals and immunostimulants like vitamin C, zinc, and beta glucan
Condition Specific Probiotic Formulas from Deerland
Deerland works with supplement brand marketers to develop these types of condition-specific probiotic formulas for sleep, mood and immune health. Learn more about our products today.
Deerland Probiotics and Enzymes has announced the publication of a human clinical trial demonstrating the synbiotic benefits of PreforPro®, a unique and proprietary blend of bacteriophages, in promoting the balance of good bacteria in the microbiome.
The goal of the parallel-arm study was to investigate whether adding bacteriophages with a probiotic would provide additional benefits to gastrointestinal function as well as augment the microbiota in the gut compared to the probiotic by itself, suggesting a truly synergistic capability.
The study, “PHAGE-2 Study: Supplemental Bacteriophages Extend Bifidobacterium animalis subsp. lactis BL04 Benefits on Gut Health and Microbiota”, published in Nutrients, was a randomized, double-blind, placebo-controlled clinical intervention trial. In this study, 66 participants were randomly assigned to one of three groups: placebo; Bifidobacterium animalis BL04 (B. lactis BL04) 1 billion CFU; or B. lactis BL04 (1 billion CFU) plus PreforPro (15mg). Participants consumed one capsule per day for four weeks. The outcomes sought were impacts on GI discomfort as well as changes or modulation of the gut microbiota.
Overall, the researchers found that the individuals in the B. lactis plus PreforPro group showed the following:
- Improvements in self-reported GI inflammation symptoms
- Reduction in colonic cramp discomfort
- Greater increase in the presence of Lactobacillus vs. placebo
- Decrease of strains Citrobacter and Desulfovibrio, which are associated with gut inflammation and GI disorders
- Reduction in E. coli
In conclusion, the study demonstrates that supplementing B. lactis BL04 with PreforPro can significantly modulate gut ecology to favor beneficial bacteria. “In addition, a lack of global changes to the microbiota (dysbiosis) in combination with specific modulation of certain taxa, like Lactobacillus, suggests that PreforPro displays prebiotic-like effects and may extend the GI benefits of consuming B. lactis or other probiotics.,” says John Deaton, vice president of science and technology for Deerland Probiotics & Enzymes.
“This study demonstrates the opportunity and capability of phages in the robust support of healthy microflora balance and the associated physiological benefits. Phages are clearly up-and-coming supplements in the rapidly burgeoning arena of microbiome support.”
Virtually visit Deerland Probiotics & Enzymes at our digital showroom at the 2020 Petfood Forum Connect!
In our digital showroom, you can learn more our microbiome health solutions, watch videos on how our probiotics, prebiotics and enzymes work, and speak directly with our staff for further discussion and questions. We look forward to meeting with you to learn more about your formulation needs and discuss how we can collaborate to add functional value to your pet applications.