More than 20 studies have been performed to confirm the safety and efficacy of PreforPro. In-vitro and in-vivo tests have demonstrated the growth-promoting effect of PreforPro on beneficial bacterial strains of Lactococcus, Lactobacillus, Bifidobacterium and Bacillus subtilis when competing with undesirable bacterial strains.

Promoting Probiotic Growth

In in vitro tests under physiological conditions, PreforPro has been shown to accelerate the growth of a broad spectrum of probiotic species, including:


  • B. bifidum
  • B. breve
  • B. animalis subsp. lactis
  • B. longum
  • L. acidophilus

  • L. paracasei
  • L. plantarum
  • L. rhamnosus
  • B. subtilis

Figures 1 & 2 show the difference in growth rate of beneficial Bacillus subtilis and Lactococcus lactis bacteria after the introduction of undesirable E.coli bacteria.

B. subtilis growth after 5 hours under physiological conditions, competing with E. coli

Functions in Both Small and Large Intestine

In-vivo growth of beneficial bacteria, B. longum, when competing with undesirable bacteria, E. coli, in different sections of the gastrointestinal tract of mice that were administered PreforPro.

Lactococcus lactis growth after 5 hours under physiological conditions, competing with E. coli 

Human Clinical Studies

In a human clinical study, PreforPro was shown to influence the microbial population of the gut, while also producing positive effects on cholesterol and immune response. Participants were administered one 15mg PreforPro capsule daily. When compared to the placebo group, results of the study indicated:

  • An increase in beneficial bacteria, including Bifidobacterium bifidum and Lactobacillus delbrueckii
  • Increase in counts of butyrate-producing Eubacteria
  • Decrease in taxa related to Clostridium perfringens
  • A significant decrease in the allergy-inducing interleukin 4 (IL-4) cytokine

In a second human clinical study to investigate whether adding PreforPro with a probiotic would provide additional benefits to gastrointestinal function as well as augment the microbiota in the gut compared to the probiotic by itself, the researchers found that participants consuming B. lactis plus PreforPro showed the following:

  • Improvements in self-reported GI inflammation symptoms
  • Reduction in colonic cramp discomfort
  • Greater increase in the presence of Lactobacillus vs. placebo
  • Decrease of strains Citrobacter and Desulfovibrio, which are associated with gut inflammation and GI disorders
  • Reduction in E. coli